Mouse Cluster Of Differentiation 8a ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Cluster Of Differentiation 8a in biofluid samples, such as serum, plasma, tissue homogenates or other biological fluids.This is a sandwich ELISA in strip well format with reagents for up to 96 tests. CD8a present in samples reacts with an anti-Mouse CD8a capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-CD8a detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active CD8a in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse CD8a. Detection Target: Mouse Cluster Of Differentiation 8a Reactivity: Mouse Range: 78.1-5000pg/mL Sensitivity: 32.6pg/mL Storage Conditions: Store all components at 4┬░C This Mouse CD8a activity assay is for the quantitative determination of Cluster Of Differentiation 8a in samples including serum, plasma, tissue homogenates or other biological fluid For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.