Mouse Tumor Necrosis Factor Beta ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Tumor Necrosis Factor Beta in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . TNFb present in samples reacts with an anti-Mouse TNFb capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-TNFb detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active TNFb in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse TNFb. Detection Target: Mouse Tumor Necrosis Factor Beta Reactivity: Mouse Range: 15.6-1000pg/mL Sensitivity: 5.7pg/mL Storage Conditions: Store all components at 4┬░C This Mouse TNFb activity assay is for the quantitative determination of Tumor Necrosis Factor Beta in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.